CheKine™ γ-谷氨酰半胱氨酸连接酶（GCL）活性检测

CheKine™ γ-谷氨酰半胱氨酸连接酶（GCL）活性检测试剂盒（微量法）

货号：KTB1680

产品应用：细胞类代谢研究 | 检测类型：氨基酸代谢 | 检测方法：比色法

核心产品特性

• 广泛样本兼容：适用于组织、细胞、细菌、血清及动植物样本的GCL活性检测。
• 标准化操作支持：含标准品与标准曲线，提供样品制备指南及酶活计算方案。
• 微量高效：48T/48S与96T/96S双规格可选，适配不同实验通量需求。

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Standard

实验关键提示

• 样本处理全程需冰上操作，匀浆液避免反复冻融，酶活测定须当日完成。
• 禁止混用不同批次或厂家的组分，更换吸头防止交叉污染。
• 复溶组分时避免气泡产生，实验前确认温度条件符合要求。

存储与运输

保存条件：-20℃避光保存，有效期6个月（自收货日起）
运输方式：冰袋（蓝冰）低温运输

产品背景

GCL为谷胱甘肽（GSH）合成的限速酶，其活性受氧化剂、生长因子等多因素调控，直接影响GSH含量及平衡。

订购信息

规格1：48 T/48 S | 价格：¥378 | 货期：现货（次日发货）
规格2：96 T/96 S | 价格：¥598 | 货期：现货（次日发货）

警告：本产品仅限研究使用，不适用于人类或临床诊断。使用须遵守相关专利及法规，厂商不承担违规责任。

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

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CheKine™ γ-Glutamylcysteine Ligase (GCL) Activity Assay Kit (Micro Method)

Catalog Number:KTB1680

Application:Cell Metabolism Research | Assay Type:Amino Acid Metabolism | Assay Method:Colorimetric Method

Key Product Features

• Broad Sample Compatibility:Suitable for GCL activity detection in tissues, cells, bacteria, serum, and plant/animal samples.
• Standardized Operation Support:Includes standards and standard curves, provides sample preparation guidelines and enzyme activity calculation schemes.
• Micro and High Efficiency:Available in 48T/48S and 96T/96S formats to meet different experimental throughput needs.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Standard

Critical Experimental Tips

• Perform sample processing on ice, avoid repeated freeze-thaw cycles of homogenates, and complete enzyme activity assays on the same day.
• Do not mix components from different batches or manufacturers, and change pipette tips to prevent cross-contamination.
• Avoid bubble formation when reconstituting components, and ensure temperature conditions are appropriate before starting the experiment.

Storage and Shipping

Storage Conditions:Store at -20°C protected from light, valid for 6 months (from the date of receipt)
Shipping Method:Low-temperature shipping with ice packs (blue ice)

Product Background

GCL is the rate-limiting enzyme in glutathione (GSH) synthesis, and its activity is regulated by factors such as oxidants and growth factors, directly affecting GSH levels and the balance.

Ordering Information

Specification 1:48 T/48 S | Price:¥378 | Lead Time:In stock (shipped the next day)
Specification 2:96 T/96 S | Price:¥598 | Lead Time:In stock (shipped the next day)

Warning:This product is for research use only, not for human or clinical diagnosis. Use must comply with relevant patents and regulations, and the manufacturer is not responsible for non-compliant use.

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Note: The above information may change due to product optimization and upgrades. For specific information, please consult Yakeyin Company.