CheKine™ 乙酸激酶（ACK）活性检测试剂盒（微量法）

CheKine™ 乙酸激酶（ACK）活性检测试剂盒（微量法）

产品基础信息

货号：KTB1127

产品分类：核心产品（细胞分析）

检测方法：比色法

样本类型：动植物组织、细胞或细菌、血清（浆）或其他液体

产品规格与订购

• 规格1：48 T/48 S | 价格：1558元 | 货期：现货（次日发货）
• 规格2：96 T/96 S | 价格：2598元 | 货期：现货（次日发货）

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ

特点与优势

验证的样本类型齐全，操作流程简捷高效。

保存与运输

保存建议：-20℃避光保存，有效期6个月

运输条件：冰袋运输（蓝冰）

注意事项

• 推荐使用新鲜样本，如需保存请置于-80℃，解冻后需在4 h内完成检测。
• 实验前确保所有组分及设备处于适宜温度。

检测原理

基于ACK催化乙酸与ATP生成乙酰磷酸和ADP，结合PK与乳酸脱氢酶级联反应，通过340 nm处NADH氧化速率定量ACK活性。

应用背景

乙酸激酶（ACK）是微生物碳代谢及能量代谢关键酶，在古细菌甲烷合成代谢中发挥中枢作用。

警告：

本产品仅限研究使用，不适用于人类或临床诊断。使用前请确保符合相关专利及法规要求，我方不承担因使用导致的侵权或违规责任。

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

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CheKine™ Acetate Kinase (ACK) Activity Assay Kit (Micro Method)

Basic Product Information

Catalog Number:KTB1127

Product Category:Core Products (Cell Analysis)

Assay Method:Colorimetric Method

Sample Type:Animal and Plant Tissues, Cells or Bacteria, Serum (Plasma) or Other Liquids

Product Specifications and Ordering

• Specification 1:48 T/48 S | Price:1558 RMB | Lead Time:In Stock (Shipped Next Day)
• Specification 2:96 T/96 S | Price:2598 RMB | Lead Time:In Stock (Shipped Next Day)

Kit Components

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ

Features and Advantages

Comprehensive validated sample types, simple and efficient operation process.

Storage and Shipping

Storage Recommendation:Store at -20°C protected from light, valid for 6 months

Shipping Conditions:Shipped with ice packs (blue ice)

Precautions

• Fresh samples are recommended. If storage is necessary, store at -80°C, and complete the assay within 4 h after thawing.
• Ensure all components and equipment are at appropriate temperatures before the experiment.

Assay Principle

Based on the ACK-catalyzed reaction of acetate and ATP to generate acetyl phosphate and ADP, combined with the cascade reaction of pyruvate kinase and lactate dehydrogenase, the ACK activity is quantified by the oxidation rate of NADH at 340 nm.

Application Background

Acetate kinase (ACK) is a key enzyme in microbial carbon metabolism and energy metabolism, playing a central role in the methanogenic metabolism of archaea.

Warning:

This product is for research use only and is not intended for human or clinical diagnosis. Ensure compliance with relevant patents and regulations before use. We are not responsible for any infringement or violation caused by the use of this product.

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Note: The above information may change due to product optimization and upgrades. For specific information, please consult Yakeyin Company.