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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1
- 形态:
请咨询
- 保存条件:
Store at -20°C
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
请咨询
- 级别:
仅用于科研
- 库存:
1 week
- 宿主:
Mouse
- 浓度:
请咨询
- 靶点:
REG1 alpha
- 规格:
100ul
| 背景 | This gene is a type I subclass member of the Reg gene family. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. Reg family members REG1B, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication. [provided by RefSeq, Jul 2008] |
|---|---|
| 别名 | ICRF; P19; PSP; PSPS; PSPS1; PTP; REG |
| 推荐稀释比 | ELISA 1:5000-10000 |
| 组分&重悬 | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| 纯化 | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Uniprot ID | P05451 |
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文献和实验7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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