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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2b
- 形态:
请咨询
- 保存条件:
Store at -20°C
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
请咨询
- 级别:
仅用于科研
- 库存:
1 week
- 宿主:
Mouse
- 浓度:
请咨询
- 靶点:
Rb
- 规格:
100ul
| 背景 | The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008] |
|---|---|
| 别名 | OSRC; p105-Rb; pp110; PPP1R130; pRb; RB |
| 推荐稀释比 | WB 1:2000, IHC 1:500 |
| 组分&重悬 | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| 纯化 | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Uniprot ID | P06400 |
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文献和实验7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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