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Anti-UBE2E3 OTI7E8;Mouse mAb

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  • 询价
  • DIMA
  • DME905731
  • 2025年07月12日
  • Mouse
  • Human, Dog, Rat, Monkey, Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 亚型

      IgG1

    • 形态

      请咨询

    • 保存条件

      Store at -20°C

    • 标记物

      Unconjugated

    • 适应物种

      Human, Dog, Rat, Monkey, Mouse

    • 保质期

      请咨询

    • 级别

      仅用于科研

    • 库存

      1 week

    • 宿主

      Mouse

    • 浓度

      请咨询

    • 靶点

      UBE2E3

    • 规格

      100ul

    背景 The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
    别名 UBCH9; UbcM2
    推荐稀释比 WB 1:500~2000, IHC 1:150, FLOW 1:100
    组分&重悬 PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
    纯化 Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
    Uniprot ID Q969T4

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    图标文献和实验
    相关实验
    • T-Cell Activation Using mAb to CD3

      splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16

    • Purification of mAb (IgG)

        Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers

    • Immunohistochemistry using Anti-Ganglioside Antibodies

      supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab

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