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文献和实验Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
an alternate approach, the Nucleic Acid Programmable Protein Array (NAPPA) is constructed by spotting protein?encoding plasmid DNA at high density, in addressable fashion, on an array surface. Proteins are subsequently generated in situ
into protein glycans and subsequent ligation with fluorescent azide-reactive detection probes utilizing the copper (I)-catalyzed cycloaddition reaction between azides and alkynes, or “click” chemistry. Metabolic labeling of cell surface O- linked or sialic acid
Introduction of human 14-3-3 proteins
association of 14-3-3 proteins with Cbl14-3-3 binding sequence in cytoplasmic domain of adhesion receptor,platelet glycoprotein Ib alpha--------------------------------------------------------------------------------14-3-3 brain protein homologs in plantsThe
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