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文献和实验Targeting Antipeptide Antibodies Toward Cytochrome P450 Enzymes
An approach to raising antibodies is described that can be applied to the majority of cytochrome P450 (P450) enzymes. Its application is limited only by the availability of suitable protein sequence information. The method is based
In Vitro CYP Induction in Human Hepatocytes
from nontransplantable human liver followed by their culture and treatment with new chemical entities and/or known inducers of cytochrome P450. Enzyme induction in cultured human hepatocytes can be assessed by measuring the levels of messenger ribonucleic acid
Protocols of In Vitro Protein Covalent Binding Studies in Liver
by one or more members of the cytochrome P450 family of enzymes. The resulting electrophile, if not quenched by low molecular weight endogenous nucleophiles, can form covalent adducts to cellular proteins, potentially resulting in enzyme inactivation, cell death
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