N9303771珀金埃尔默标准溶液Iron 1,000 礸/

Quick and Easy Isolation of Genomic DNA from Yeast

  Procedure Transfer 1.5 ml of liquid culture of yeast grown for 20 - 24 h at 30°C in YPD (1% yeast extract, 2% peptone, 2% dextrose) into a microcentrifuge tube. Pellet cells by centrifugation at 20,000 × g for 1-5 minutes. Add

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2

Analysis of Proteins using Small Format 2D Gel Electrophoresis

with 2ml of MEM containing 2% newborn calf serum (NBCS). The uninfected and virus infected HEp-2C cell monolayers are incubated for 4-5 hours at 37C before being washed twice with PBS-A. The cell monolayers are then overlaid with 0.5ml of methionine-free