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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated BC2 tag PDRKAAVSHWQQ derived from beta catenin.
- 形态:
Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
- 保存条件:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
- 克隆性:
Polyclonal
- 适应物种:
Protein of interest overexpressed wth BC2-tag
- 供应商:
Agrisera
- 宿主:
Rabbit
- 靶点:
Anti-BC2-tag - ALP conjugated (40 µg)
- 抗体英文名:
Anti-BC2-tag - ALP conjugated (40 µg)
- 抗体名:
Anti-BC2-tag - ALP conjugated (40 µg)
- 规格:
40 µg
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文献和实验Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
To fix, 1/10 volume of 37 formaldehyde is added to the culture, which is shaken for a further 40 minutes at room temperature. Cells are then recovered by centrifugation at 1400 X g for 2 minutes, and washed (i.e. gently resuspended then recovered
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