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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide 3xHis
- 形态:
Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
- 保存条件:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
- 克隆性:
Polyclonal
- 适应物种:
3xHis tagged proteins
- 供应商:
Agrisera
- 宿主:
Rabbit
- 应用范围:
Western blot (WB)
- 靶点:
Anti-His-tag | 3xHis (polyclonal) - ALP conjugated 40 µg
- 抗体英文名:
Anti-His-tag | 3xHis (polyclonal) - ALP conjugated 40 µg
- 抗体名:
Anti-His-tag | 3xHis (polyclonal) - ALP conjugated 40 µg
- 规格:
40 µg
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
To immunostain using enzyme-coupled secondary antibodies: i. Rinse slides in PBS and remove excess solution. ii. Add 40 µL of secondary antibody (e.g., anti-rabbit IgG [Fc] HRP conjugate, 1:100 dilution
Immunofluorescent Staining of Mouse and Rat Leukocytes
secondary reagent (e.g., fluorochrome-conjugated avidin, streptavidin, anti-Ig allotype, anti-Ig isotype, polyclonal anti-Ig). For example, dilute antibody to ~1 µg per 100 µl in wash buffer and add this to each well containing the loosened cell pellet
-Whittaker Europe, Verviers, Belgium) supplemented exactly as described previously [10]. B cell activation was performed using varying concentrations of a polyclonal B cell activator, i.e. anti-µ-Fab'2 fragments (Irvine Scientific, Santa Anna, CA, USA
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