- 询价
- Agrisera
- Sweden
- AS21 4695
- 2026年01月09日
- Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF)
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
Total IgG. Protein A affinity purified in 5 mM phosphate, 75 mM NaCl, ph 7.8 with 0.06% sodium azide. Contains sucrose for stabilization.
- 保存条件：
Store at 4°C or -20°C; and make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- 克隆性：
Monoclonal
- 供应商：
Agrisera
- 宿主：
Mouse
- 应用范围：
Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF)
- 靶点：
Mouse IgG negative control for ChIP
- 抗体英文名：
Mouse IgG negative control for ChIP
- 抗体名：
Mouse IgG negative control for ChIP
- 规格：
100 µg at 1 µg/µl
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文献和实验Active Motif ChIP 实验专家技术经验分享：如何判断 ChIP 成功与否？
好之后分别用 5% Input，H3K4me3 ChIP，IgG ChIP 的 DNA 作为模板，qPCR 分别扩增 A，B，C 三个区域。H3K4me3 在任何一个区域的富集都都可以用这个公式计算：5% × 2[CT value (Input-H3K4me3)]，同样 IgG 的富集度的计算公式是：5% × 2[CT value (Input-IgG)]。如下表所示：CT values5% InputIgGH3K4me3A (Target region)263227B (Negative
Chromatin Immunoprecipitation (ChIP)
by using real-time PCR to quantify the abundance of the DNA fragment of interest added to the ChIP reaction, with respect to the abundance of the DNA fragment found in the final immunoprecipitate. 2. As a critical negative control
. Mice and human with Foxp3 mutations are severely impaired in Treg cell generation and develop lethal autoimmune diseases. We combined chromatin immuno-precipitation and mouse whole genome tiling array profiling (ChIP-on-Chip) to identify the direct
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