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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
BSA-conjugated 5-Methylcytosine
- 亚型:
IgM
- 形态:
Purified IgM in PBS. Contains 50 % glycerol, filter sterilized, biotinylated.
- 保存条件:
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- 克隆性:
Monoclonal
- 适应物种:
Chlamydmonas me-1 cells, mouse embryonic stem cells
- 供应商:
Agrisera
- 宿主:
Mouse
- 应用范围:
Western blot (WB)
- 靶点:
Anti-5MeC | 5-Methylcytosine (clone 5MC-CD) biotinylated
- 抗体英文名:
Anti-5MeC | 5-Methylcytosine (clone 5MC-CD) biotinylated
- 抗体名:
Anti-5MeC | 5-Methylcytosine (clone 5MC-CD) biotinylated
- 规格:
50 µg at 1 µg/µl
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文献和实验Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
) A431 cells. At 48 h after infection, these cells were stained with ten different primary antibodies (5 g/ml) specific for different cell surface markers: anti-CD15 (clone 2F3; BD, PharMingen, San Diego, CA), anti-ERBB-2 (clone SP77; ref. 5), anti-ERBB
Immunohistochemisty-Fluorescence Protocol
and Secondary Reagents Biotinylated donkey anti-goat IgG (Jackson Immuno Research Lab Catalog # 705-066-147) Biotinylated goat anti-mouse IgG1 (Caltag Lab Catalog # M32115) FITC-labeled goat anti-mouse IgG1 (Caltag Lab Catalog # M32001)
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