B3001566珀金埃尔默塑料制样品杯Polypropyle

Sample Preparation for Microinjection

(usually takes 6–8 hr). Transfer coverslips into 35 mm petri dishes containing 2 ml of culture medium and let cells grow for 2 days at 37°C. After this time, 500 to 1000 cells will usually be in the center of the coverslip. Microinject all cells

Sample preparation (analytical gels)

): Five ml of supernatant HEPG2 culture media were concentrated down to 30 μl in a MicrosepTM Concentrators. The concentrated sample was mixed with 60 μl of a solution containing urea (8 M), CHAPS (4% w/v), Tris (40 mM), DTE (65 mM) and a trace of bromophenol

RNA AND PROTEIN EXTRACTION FROM THE SAME SAMPLE

  PROCEDURE 1. Grind frozen or fresh tissues (up to 1 g) to a fine powder using a mortar and pestle under liquid nitrogen. Add 4.5 mL of extraction buffer and 10 mL of b -mercaptoethanol. Mix with the pestle