N0777941珀金埃尔默塑料制离心管17 mm x100

DNA from Tail Biopsies

1. Remove 0.5 cm of tail into polypropylene microfuge tube (do not mince). (The tubes must have tight-fitting caps, so that there are no leaks in steps 3 and 7 below.) 2. Add 0.5 ml DNA digestion buffer with proteinase K added to 0.5 mg/ml final

DNA from Tail Biopsies

1. Remove 0.5 cm of tail into polypropylene microfuge tube (do not mince). (The tubes must have tight-fitting caps, so that there are no leaks in steps 3 and 7 below.) 2. Add 0.5 ml DNA digestion buffer with proteinase K added to 0.5 mg/ml final

Flow Cytometry of Fibroblast Nuclei for DNA content

dish with PBS (Ca++ /Mg++ free).  Cover with 1.5 mL 0.1% (2.5 mM) EDTA in PBS at 37ºC x 10 min.  Loosen cells by vigorous pipetting then transfer suspension to 1.5 mL Eppendorf tubes on ice. Spin at 1000 RPM, 4ºC.  Discard supernatant.  Resuspend