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- PerkinElmer
- N6480080
- 2025年07月16日
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
Embryonic Fibroblasts (Irradiated) 4. DMEM with GlutaMAX™-I (High Glucose) 5. KnockOut™ DMEM/F-12 6. Fetal Bovine Serum (FBS), ES Cell-Qualified 7. KnockOut™ Serum Replacement (KSR) 8. MEM Non-Essential Amino Acids (NEAA)
HIGH RESOLUTION GENETIC FOOTPRINTING
-stranded DNA binding resin (Clontech), followed by Quiaquick PCR purification kit (Quiagen). The purified PCR product is then digested with the restriction enzyme whose recognition site is unique to the replacement oligonucleotide, to generate a ladder
0.5% 0.5% 0.5% 0.5% 0.5% 0.5% 0.5% 0.5% 0.5% - 0.5% 0.5% 0.5% 0.5% FBS - - - - - - - - - 10% - - - - KnockOut Serum Replacement 20% - - - - - - - - - - - - - L-glutamine 2 mM 2 mM 2 mM 2 mM 2 mM 2 mM 2 mM 2 mM 2 mM
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