N6101251珀金埃尔默玻璃制进样针5 #L Syring

Purification of recombinant sBRF M166L

300mM NaCl 2.63 g NaCl 6.0M Gu-HCl 86 g Guanidine-HCl 10mM Imidazole 0.1 g Imidazole Wash Buffer, pH8.0 (200 ml

Purification of recombinant sBRF M166L

and/or protein assay.Typical recovery is 2 to 6 mg BRF protein. -------------------------------------------------------------------------------- Lysis/Binding buffer,pH 8.0 (150 ml) 30mM Tris 8.0 0.54 g Tris 300mM NaCl2.63 g NaCl 6.0M Gu-HCl86 g Guanidine

v468 Chapter 2 Wnt活性蛋白分离及应用

,NJ), loaded with Cu 2+ . 3. Binding Buffer: 0.5 M NaCl, 20 mM Tris-HCl, pH 7.5, and1% (w/v) CHAPS. 4. Elution Buffer: Binding Buffer with 100 mM imidazole, pH 7.5. 5. Syringe (10 mL) with 0.2-μm pore size filter. 2.3.3. Step 3: Gel