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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
(1-Methyl-1H-imidazol-2-yl)methylamine
- 库存:
98
- 供应商:
北京孚博
- CAS号:
124312-73-8
- 规格:
1g
CAS:124312-73-8
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文献和实验Small scale His-Tag fusion protein purification under denaturative conditions
of the sonicator) 4 Spin 15min max speed 4°C 5 Transfer supernatant into clean tube: crude extract (keep 40µl for PAGE-SDS) see Equilibration of Ni-NTA agarose ) 7 Add the crude extract to the beads and incubate 4°C / 1h (swirl
Critical Appraisal of the MTT Assay in the Presence of Rottlerin and Uncouplers
out to clarify the surprising lack of effect of Rottlerin on MCF-7 cell viability, assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test. We found that Rottlerin causes overestimation of the MTT test, leading to inconsistent
-4-sulfonate (15 ,16 ), methyl 4-(6-methoxynaphthalene-2-yl)-4-oxo-2-butenoate (17 ), N-(1-pyrenyl)maleimide (18 ), monobromobimane (19 –23 ), o -phthaldialdehyde (24 ,25 ), 9-fluorenylmethyloxycarbonyl chloride (26 ), and 2-chloro-1-methylpyridinium
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