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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Ethanol,2,2'-[(1-methylethyl)imino]bis-
- 库存:
98
- 供应商:
北京孚博
- CAS号:
121-93-7
- 规格:
1g
CAS:121-93-7
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文献和实验The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
Preparation of Polyacrylamide Gels
2.5 g Bis-Acrylamide 8 M Urea 422.0 g Urea 0.5 X TBE 25 mL 20X TBE 500 mL ddH20 Mix. Bring volume to 1 L. Filter. Store at 4 C in the dark. 3. Wash glass plates on both sides with soap. Rinse well with water. Rinse well with distilled water
You will need: Freshly made 2M NaOH 3M sodium acetate, pH 4.5 Sterile, distilled water Absolute ethanol 70% ethanol 7 x DNA annealing buffer (280mM Tris.Cl, pH 7.5, 100mM MgCl2, 350mM NaCl) Termination mixes (40mM Tris.Cl, pH 7.5, 50mM NaCl, 10mM MgCl2, 150mM dTTP
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