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100 ul
predicted_size:69.5 kDa
Uniprot ID:Q14145
description:KEAP1 mouse monoclonal antibody, clone OTI1G2 (formerly 1G2)
clonality:Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...):Primary Antibodies
background:This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq]
immunogen:Full length human recombinant protein of human KEAP1 (NP_987096) produced in HEK293T cell.
recommended_dilution:WB 1:2000, IF 1:100, FLOW 1:100
predicted_size:69.5 kDa
buffer:PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
host_species:Mouse
applications:FC, IF, WB
reactivities:Human, Mouse, Rat
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
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