OriGene MSH2 Rabbit Monoclonal

ProductName：MSH2 Rabbit Monoclonal Antibody [Clone ID: R03-1F3]
description：MSH2 Rabbit monoclonal Antibody
symbol：MSH2
synonyms：COCA1; FCC1; HNPCC; HNPCC1; LCFS2
locusid：4436
Concentration：lot specific
sub_category：Primary Antibodies
uniprot_id：P43246
Applications：IP, WB
CNdelivery：2周
RecommendDilution：WB: 1/1000-1/5000
IP: 1/20-1/50
Reactivity：Human
Immunogen：A synthetic peptide of human MSH2
Purification：Affinity Purified
Conjugation：Unconjugated
ProteinFamilies：null
ProteinPathways：null
GeneName：mutS homolog 2
Background：Swiss-Prot Acc.P43246.Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containg DNA strand (PubMed:26300262). ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
Buffer：50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
isotypes_filter：IgG
predicted_size：Calculated MW: 105 kDa; Observed MW: 105 kDa