Recombinant IL-34 (Interleukin

gene_symbol
Description：Recombinant IL-34 (Interleukin-34)，Mouse
Accn
Unipro ID：Q8R1R4
Synonyms：2010004A03Rik,Il34
Species：Mouse
Amount：20 ug
Delivery time：2周
Expression sequence：MNENLEIWTLTQDKECDLTGYLRGKLQYKNRLQYMKHYFPINYRIAVPYEGVLRVANITRLQKAHVSERELRYLWVLVSLNATESVMDVLLEGHPSWKYLQEVQTLLENVQRSLMDVEIGPHVEAVLSLLSTPGLSLKLVRPKALLDNCFRVMELLYCSCCKQSPILKWQDCELPRLHPHSPGSLMQCTATNVYPLSRQTPTSLPGSPSSSHGSLP with polyhistidine tag at the C-terminus.
Tags：His Tag (C-term)
PredictedMW：The protein has a calculated MW of 25.52 kDa. The protein migrates about 25 kDa under reducing condition (SDS-PAGE analysis).
Buffer：The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
Stability
Bioactivity：Measure by its ability to induce proliferation in NFS-60 cells. The ED₅₀ for this effect is 3.3 x 10⁴ IU/mg.
Purity：>98% as determined by SDS-PAGE.
Concentration
Preparation
Endotoxin：<0.1 EU per 1 μg of the protein by the LAL method.
Shipping
Background：Interleukin 34 (IL-34) is a 23.98 kDa cytokine with 216 amino acid residues. IL-34 is a ligand of the colony-stimulating factor 1 receptor (CSF-1R) and is mainly secreted from the adrenal, frontal lobe, and cortex. IL-34 play a vital role in the proliferation, survival, differentiation, and migration of myeloid cells. These functions are mediated by IL-33 activating ERK1 /2, JAK, and PI3K/AKT signal pathways.