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Description:Recombinant IL-17F (Interleukin-17F),Mouse
Accn
Unipro ID:Q7TNI7
Synonyms:C87042
Species:Mouse
Amount:20 ug
Delivery time:2周
Expression sequence:MRKNPKAGVPALQKAGNCPPLEDNTVRVDIRIFNQNQGISVPREFQNRSSSPWDYNITRDPHRFPSEIAEAQCRHSGCINAQGQEDSTMNSVAIQQEILVLRREPQGCSNSFRLEKMLLKVGCTCVKPIVHQAA with polyhistidine tag at the C-terminus.
Tags:His Tag (C-term)
PredictedMW:The protein has a calculated MW of 15.82 kDa. The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
Buffer:The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, pH 4.5.
Stability
Bioactivity:Measure by its ability to induce IL-6 secretion in 3T3 cells. The ED₅₀ for this effect is <100 ng/mL.
Purity:>98% as determined by SDS-PAGE.
Concentration
Preparation
Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.
Shipping
Background:Interleukin 17F (IL-17F) predicts a molecular mass of 30.1 kDa, is a cytokine of innate and adaptive immune system involved in maintenance of tissue integrity and antimicrobial host defense against infection by inducing the expression of genes that encode other proinflammatory cytokines, such as tumor necrosis factor, interleukin 1, interleukin 6 and some members of the colony-stimulating factor family.
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文献和实验【进展|热点】Immunity:IL-17A和IL-17F的生物效应不同
IL-17RA和IL-17RC的表达也具有组织特异性,分别采用IL-17A和IL-17F刺激直肠上皮细胞、巨噬细胞和T细胞等,作者发现这些细胞接受IL-17A和IL-17F刺激后,细胞因子分泌谱不同,这表明IL-17A和IL-17F的生物学功能确实存在差异。 Immunity在同期发表了针对该研究的述评。 Ishigame H, Kakuta S, Nagai T, et al. Differential roles of interleukin-17A and -17F in host
【进展|热点】Immunity:IL-17A和IL-17F的表达调控机制不同
IL-17A和IL-17F是Th17细胞的主要效应因子,在很长的一段时间内,人们一直认为二者结构高度同源,且受体相同,因此可能生物效应和表达调控机制也相同,在文献资料以及实验中并未对二者进行严格区分。 Immunity在2月份曾发表文章,指出IL-17A和IL-17F的生物学效应略有不同。 连接: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSP-4VCPJNP-D&_user=2352871&_rdoc
Lectin Glycoprofiling of Recombinant Therapeutic Interleukin-7
glycoproteins and particularly relevant for a first study of lot-to-lot comparison, or detection of unwanted glycans. In this chapter, we describe a lectin array-type method specifically designed for the study of recombinant therapeutic interleukin-7 (rhIL
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