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Description:Recombinant IL-5 (Interleukin-5),Human
Accn
Unipro ID:P05113
Synonyms:EDF, BCDFII, TRF
Species:Human
Amount:20 ug
Delivery time:2周
Expression sequence:MIPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES with polyhistidine tag at the C-terminus.
Tags:His Tag (C-term)
PredictedMW:The protein has a calculated MW of 14.09 kDa. The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis).
Buffer:The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.
Stability
Bioactivity:Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <0.3 ng/mL.
Purity:>98% as determined by SDS-PAGE.
Concentration
Preparation
Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.
Shipping
Background:IL-5 is a 45-60 kDa homodimer cytokine that functions principally in the eosinophil arm of the Th2 response. It is a member of the helical bundle family of cytokines, along with IL-3, IL-4, and granulocyte-macrophage colony-stimulating factor (GM-CSF).
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文献和实验Lectin Glycoprofiling of Recombinant Therapeutic Interleukin-7
glycoproteins and particularly relevant for a first study of lot-to-lot comparison, or detection of unwanted glycans. In this chapter, we describe a lectin array-type method specifically designed for the study of recombinant therapeutic interleukin-7 (rhIL
白细胞介素 IL-5 IL-5主要产生细胞:活化T细胞(Th2)、嗜酸粒细胞(同源二聚体) IL-5氨基酸数目:115 IL-5分子质量(kDa):35-60 IL-5受体:CDl25 IL-5主要生物学活性:诱导小鼠B细胞分化为抗体分泌细胞、IgA合成;协同11-2促进CTL分化;促进人嗜酸粒细胞激活、增殖、分化和趋化
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人白介素-5 (IL-5)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 IL-5 单抗包被于酶标板上,标准品和样品中的 IL-5与单抗结合,加入生物素化的抗人 IL-5
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