Recombinant CD27L (CD27 ligand

gene_symbol
Description：Recombinant CD27L (CD27 ligand)，Human
Accn
Unipro ID：P32970
Synonyms：soluble CD27 Ligand, sCD27 Ligand, TNFSF7, CD70
Species：Human
Amount：20 ug
Delivery time：2周
Expression sequence：QRFAQAQQQLPLESLGWDVAELQLNHTGPQQDPRLYWQGGPALGRSFLHGPELDKGQLRIHRDGIYMVHIQVTLAICSSTTASRHHPTTLAVGICSPASRSISLLRLSFHQGCTIASQRLTPLARGDTLCTNLTGTLLPSRNTDETFFGVQWVRP with polyhistidine tag at the N-terminus
Tags：His Tag (N-term)
PredictedMW：The protein has a calculated MW of 18.08 kDa. The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Buffer：The protein was lyophilized from a 0.2 µm filtered solution containing containing 0.05% sarkosyl, in 1X PBS, pH 8.0.
Stability
Bioactivity：Measure by its ability to induce IL-8 secretion in human PBMCs. The ED₅₀ for this effect is <0.6 ng/mL.
Purity：>98% as determined by SDS-PAGE.
Concentration
Preparation
Endotoxin：<0.1 EU per 1 μg of the protein by the LAL method.
Shipping
Background：Human CD27L is also named for CD70 which is one member of TNF family. Human CD27L is expressed on T and B lymphocytes and mature DCs. It binds the CD27, which is on the antigen-presenting cells. Human CD27L is a 23 kDa cytokine with 154 amino acid residues which is a transmembrane glycoprotein. Human CD27L plays an important role in the regulation of T cell proliferation which is also a good target of cancer immunotherapy.