Recombinant CCL3 (C-C motif ch

gene_symbol
Description：Recombinant CCL3 (C-C motif chemokine ligand 3)，Human
Accn
Unipro ID：P10147
Synonyms：MIP-1a: Macrophage Inflammatory Protein-1α, LD78α
Species：Human
Amount：20 ug
Delivery time：2周
Expression sequence：ADTPTACCFSYTSRQIPQNFIADYFETSSQCSKPGVIFLTKRSRQVCADPSEEWVQKYVSDLELSA with polyhistidine tag at the N-terminus
Tags：His Tag (N-term)
PredictedMW：The protein has a calculated MW of 8.4 kDa. The protein migrates as 8-11 kDa under reducing condition (SDS-PAGE analysis).
Buffer：The protein was lyophilized from a 0.2 µm filtered solution containing 1×PBS, pH 8.5.
Stability
Bioactivity：Measure by its ability to chemoattract human PBMC using a concentration range of 5 -50 ng/mL.
Purity：>95% as determined by SDS-PAGE.
Concentration
Preparation
Endotoxin：<0.1 EU per 1 μg of the protein by the LAL method.
Shipping
Background：C-C Motif Chemokine Ligand 3 (CCL3) is a 7.33Da cytokine with 66 amino acid residues. CCL3 is also called macrophage inflammatory protein 1-alpha (MIP-1-alpha) and is expressed in bone marrow and liver. Upon binding to the receptor, CCR1, CCR4, or CCR5, CCL3 plays an essential role in acute inflammatory responses like recruitment and activation of polymorphonuclear leukocytes and induction of monophasic fever. In addition, CCL3 participates in resistance to type 1 virus infection, mediation of MAPK signaling pathway, cell-cell signaling, and calcium-mediated signaling.