Recombinant BDNF (Brain-derive

gene_symbol
Description：Recombinant BDNF (Brain-derived neurotrophic factor)，Human/Mouse/Rat
Accn
Unipro ID：P23560
Synonyms：ANON2, BULN2
Species：Human/Mouse/Rat
Amount：20 ug
Delivery time：2周
Expression sequence：MHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR with polyhistidine tag at the C-terminus.
Tags：His Tag (C-term)
PredictedMW：The protein has a calculated MW of 14.45 kDa. The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis).
Buffer：The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Stability
Bioactivity：Measure by its ability to induce proliferation in BaF3 cells transfected with TrkB. The ED₅₀ for this effect is <2 ng/mL.
Purity：>98% as determined by SDS-PAGE.
Concentration
Preparation
Endotoxin：<0.1 EU per 1 μg of the protein by the LAL method.
Shipping
Background：Brain-derived neurotrophic factor (BDNF) is a member of neurotrophin family that not only primarily expressed in hippocampus, amygdala, cerebral cortex, hypothalamus and cerebellum but also has been detected in blood platelets and in circulating plasma. BDNF is a 27.8 kDa protein containing 247 residues, which plays a critical role in regulating synaptic transmission and plasticity in various region of the CNS. Additionally, BNDF can acts as a modulator in the long-term potentiation of memory-related modifications in hippocampal synaptic transmission.