Purified recombinant protein o

gene_symbol：Glutathione Peroxidase 4
Description：Purified recombinant protein of Human glutathione peroxidase 4 (phospholipid hydroperoxidase) (GPX4), transcript variant 1, Gly73-End, with N-terminal His tag, expressed in E.coli, 50ug
Accn：NM_002085
Unipro ID：P36969
Synonyms：GPx-4; GSHPx-4; MCSP; PHGPx; SMDS; snGPx; snPHGPx
Species：Human
Amount：50 ug
Delivery time：现货
Expression sequence：A DNA sequence encoding the region(Gly73-End) of GPX4
Tags：N-His
PredictedMW：14.2 kDa
Buffer：50 mM Tris-HCl, pH 8.0, 8 M urea
Stability：Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity
Purity：> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration：>0.05 µg/µL as determined by microplate BCA method
Preparation
Endotoxin
Shipping
Background：The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization. [provided by RefSeq, Dec 2018]