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Description:Recombinant protein of human programmed cell death 1 ligand 2 (PDCD1LG2), 20 µg
Accn:NM_025239
Unipro ID:Q9BQ51
Synonyms:B7DC; bA574F11.2; Btdc; CD273; PD-L2; PDCD1L2; PDL2
Species:Human
Amount:20 ug
Delivery time:现货
Expression sequence:>RC224141 representing NM_025239 Red=Cloning site Green=Tags(s) MIFLLLMLSLELQLHQIAALFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHR ERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVE LTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQ SQMEPRTHPTWLLHIFIPSCIIAFIFIATVIALRKQLCQKLYSSKDTTKRPVTTTKREVNSAI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tags:C-Myc/DDK
PredictedMW:30.8 kDa
Buffer:25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Stability:Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>0.05 µg/µL as determined by microplate BCA method
Preparation:Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Endotoxin
Shipping
Background:Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity).[UniProtKB/Swiss-Prot Function]
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文献和实验Ligand Characterization Using Microphysiometry
the use of a Cytosensor microphysiometer for functional characterization of an agonist and antagonist to a G protein?coupled receptor, the muscarinic M1 receptor. Concentration?response profiles are used to calculate values for the EC50 of the response
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
Wash buffer (see recipe ) 20 µg each of the bait proteins at 400 ng/µl (see protocol 2 ) Dilution
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