Recombinant protein of human v

gene_symbol：RAF1
Description：Recombinant protein of human v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1),residues Ser306-Phe648, with N-terminal polyhistidine tag,expressed in sf9 cells
Accn：NM_002880
Unipro ID：P04049
Synonyms：c-Raf; CMD1NN; CRAF; NS5; Raf-1
Species：Human
Amount：20 ug
Delivery time：现货
Expression sequence：A DNA sequence from TrueORF clone, [RC201983], encoding the region(Ser306-Phe648) of human RAF1
Tags：N-His
PredictedMW：39 kDa
Buffer：50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 20% glycerol
Stability：Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity
Purity：> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration：>0.05 µg/µL as determined by microplate BCA method
Preparation
Endotoxin
Shipping
Background：This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]