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Description:Recombinant protein of human metastasis associated 1 (MTA1), 20 µg
Accn:NM_004689
Unipro ID:Q13330
Synonyms
Species:Human
Amount:20 ug
Delivery time:现货
Expression sequence:>RC218599 representing NM_004689 Red=Cloning site Green=Tags(s) MAANMYRVGDYVYFENSSSNPYLIRRIEELNKTANGNVEAKVVCFYRRRDISSTLIALADKHATLSVCYK AGPGADNGEEGEIEEEMENPEMVDLPEKLKHQLRHRELFLSRQLESLPATHIRGKCSVTLLNETESLKSY LEREDFFFYSLVYDPQQKTLLADKGEIRVGNRYQADITDLLKEGEEDGRDQSRLETQVWEAHNPLTDKQI DQFLVVARSVGTFARALDCSSSVRQPSLHMSAAAASRDITLFHAMDTLHKNIYDISKAISALVPQGGPVL CRDEMEEWSASEANLFEEALEKYGKDFTDIQQDFLPWKSLTSIIEYYYMWKTTDRYVQQKRLKAAEAESK LKQVYIPNYNKPNPNQISVNNIKAGVVNGTGAPGQSPGAGRACESCYTTQSYQWYSWGPPNMQCRLCASC WTYWKKYGGLKMPTRLDGERPGPNRSNMSPHGLPARSSGSPKFAMKTRQAFYLHTTKLTRIARRLCREIL RPWHAARHPYLPINSAAIKAECTARLPEASQSPLVLKQAVRKPLEAVLRYLETHPRPPKPDPVKSVSSVL SSLTPAKVAPVINNGSPTILGKRSYEQHNGVDGNMKKRLLMPSRGLANHGQTRHMGPSRNLLLNGKSYPT KVRLIRGGSLPPVKRRRMNWIDAPDDVFYMATEETRKIRKLLSSSETKRAARRPYKPIALRQSQALPPRP PPPAPVNDEPIVIED TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tags:C-Myc/DDK
PredictedMW:80.6 kDa
Buffer:25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Stability:Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>0.05 µg/µL as determined by microplate BCA method
Preparation:Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Endotoxin
Shipping
Background:This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
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文献和实验Introduction of human 14-3-3 proteins
of their association with the G-box DNA/protein complex by a monoclonal antibody screening approach.GF14 proteins are homologous to the 14-3-3 family of mammalian proteins.Here we demonstrate that recombinant GF14 omega,one member of the Arabidopsis GF14 protein family
Overview of Protein Microarrays
1:189‐203. Chao, G., Lau, W.L., Hackel, B.J., Sazinsky, S.L., Lippow, S.M., and Wittrup, K.D. 2006. Isolating and engineering human antibodies using yeast surface display. Nat
Strategies to Optimize Protein Expression in E. coli
. 2009a. Recombinant expression and affinity purification of a novel epididymal human sperm‐binding protein, BSPH1. Mol. Hum. Reprod. 15:105‐114. Lefebvre, J., Boileau, G
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