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Description:Recombinant protein of human sigma non-opioid intracellular receptor 1 (SIGMAR1), transcript variant 1, 20 µg
Accn:NM_005866
Unipro ID:Q99720
Synonyms:ALS16; DSMA2; hSigmaR1; OPRS1; SIG-1R; sigma1R; SR-BP; SR-BP1; SRBP
Species:Human
Amount:20 ug
Delivery time:现货
Expression sequence:>RC201206 protein sequence Red=Cloning site Green=Tags(s) MQWAVGRRWAWAALLLAVAAVLTQVVWLWLGTQSFVFQREEIAQLARQYAGLDHELAFSRLIVELRRLHP GHVLPDEELQWVFVNAGGWMGAMCLLHASLSEYVLLFGTALGSRGHSGRYWAEISDTIISGTFHQWREGT TKSEVFYPGETVVHGPGEATAVEWGPNTWMVEYGRGVIPSTLAFALADTVFSTQDFLTLFYTLRSYARGL RLELTTYLFGQDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tags:C-Myc/DDK
PredictedMW:24.9 kDa
Buffer:25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Stability:Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>0.05 µg/µL as determined by microplate BCA method
Preparation:Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Endotoxin
Shipping
Background:This gene encodes a receptor protein that interacts with a variety of psychotomimetic drugs, including cocaine and amphetamines. The receptor is believed to play an important role in the cellular functions of various tissues associated with the endocrine, immune, and nervous systems. As indicated by its previous name, opioid receptor sigma 1 (OPRS1), the product of this gene was erroneously thought to function as an opioid receptor; it is now thought to be a non-opioid receptor. Mutations in this gene has been associated with juvenile amyotrophic lateral sclerosis 16. Alternative splicing of this gene results in transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2013]
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文献和实验Recombinant Opioid Receptors: Structure-Function Relationship
-terminus, seven hydrophobic transmembrane domains (TM1-7), connected by relatively short intracellular and extracellular loops, and a short intracellular C-terminal tail. Comparison of their deduced protein sequences revealed that opioid receptors
Intracellular Cytokine Staining Protocol
that the investigators evaluate the use and efficacy of different protein transport inhibitors in their specific assay system.Note: Generally, the non-specific background staining of fixed and permeabilized cells is higher than surface staining; therefore, extra protein
Intracellular Immunofluorescent Staining for Flow Cytometry
Annotations: Iono=Ionomycin; PMA=Phorbol Myristate Acetate; LPS=Lipopolysaccharide; 2d=2 day culture; 5hr=5 hour culture; LPS for activation of human PBMC obtained from Sigma (#L-8274) Table 3: Non-Cytokine Proteins
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