Recombinant protein of human e

gene_symbol：elF2 alpha
Description：Recombinant protein of human eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa (EIF2S1), 20 µg
Accn：NM_004094
Unipro ID：P05198
Synonyms：EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A
Species：Human
Amount：20 ug
Delivery time：4周
Expression sequence：>RC200368 protein sequence Red=Cloning site Green=Tags(s) MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNEC VVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDD KYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKE ALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTD TDETELARQMERLERENAEVDGDDDAEEMEAKAED TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tags：C-Myc/DDK
PredictedMW：35.9 kDa
Buffer：25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Stability：Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity
Purity：> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration：>0.05 µg/µL as determined by microplate BCA method
Preparation：Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Endotoxin
Shipping
Background：The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]