1,3,3-trimethylbicyclo[2.2.1]h

SPRi在核酸适配体开发中的应用

at the 3’ end from Sigma-Aldrich.The (1-mercaptoundec-11-yl)tetra(ethylene glycol) (MUTEG) was purchased from Sigma-Aldrich. Immobilization of the human IgE (hIgE) aptamer on the SPRi-Biochip™ The thiol modified IgE aptamer and reference

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

  Figure 4.52.2 Preparation of 5′‐ O ‐aminooxymethyl‐2′‐deoxythymidine (S.10 ). Abbreviations: Lev, levulinyl; Thy, thymin‐1‐yl; DBU, 1,8‐diazabicyclo[5.4.0]undec‐7‐ene. Adapted from Cieślak et al

Release of DNA Oligonucleotides and Their Conjugates from Controlled‐Pore Glass Under Thermolytic Conditions

Additional reagents and equipment for quantitative trityl assay (unit 3.2 ), automated oligonucleotide synthesis ( appendix 3C ), and polyacrylamide gel electrophoresis (unit 10.4 and appendix 3B