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Size：1 x 96 wells
Curve Range：62.5-4000pg/mL
Specificity：This kit is used for quantitative detection of PIP5K3
Sensitivity：22pg/mL
Reactivity：Human
Cross Reactivity：There is no detectable cross-reactivity with other relevant proteins.
Components：PIP5K3 Antibody Coated 96-well Plate in foil pouch with desiccant|1 platePIP5K3 Standard Protein|0.1 mLPIP5K3 Detection Antibody|0.12 mL 1x SA-HRP Conjugate|0.12 mLAssay Buffer|40 mLSample Diluent|30 mLWash Buffer Concentrate 20X|60 mLTMB Substrate|14 mLStop Solution|14 mL
Gene Symbol：PIKFYVE
Species：Human
Deliveryn Time：2周
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文献和实验Keywords: human embryonic stem cells; mouse neural stem cells; MTT assay; 96?well plate assays; toxicology GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online
is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
Activation and Expansion of Human Treg Cells
of Human Treg Cells Day 0: Start with 1 x 105 Treg cells in 100 μl Culture Medium in a round bottom 96 well tissue culture plate. Add 20 μl Dynabeads Human Treg Expander. Add 500 U/ml rIL-2. Day 1: Add 100 μl Culture Medium containing 500 U/ml
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