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- OriGene
- EA100202
- 2025年12月26日
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Size：1 x 96 wells
Curve Range：15.6pg/ml-1000pg/ml
Specificity：This kit is used for quantitative detection of rat Activin A
Sensitivity：<12pg/ml
Reactivity：Rat
Cross Reactivity：There is no detectable cross-reactivity with other relevant proteins.
Components： 96-well plate precoated with anti- rat Activin A antibody 1Lyophilized recombinant rat Activin A standard 10ng/tubex2Biotinylated anti- rat Activin A antibody 130ul(dilution 1:100)Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)Sample diluent buffer 30mlAntibody diluent buffer 12mlABC diluent buffer 12mlTMB color developing agent 10mlTMB stop solution 10ml
Gene Symbol：Inhba
Species：Rat
Deliveryn Time：3周
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
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