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30 ul
predicted_size:26.4 kDa
Uniprot ID:P49771
description:Anti-FLT3LG (Flt3 ligand) mouse monoclonal antibody, clone OTI8D10 (formerly 8D10)
clonality:Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...):Primary Antibodies
background:Stimulates the proliferation of early hematopoietic cells. Synergizes well with a number of other colony stimulating factors and interleukins
immunogen:Recombinant protein expressed in E.coli corresponding to amino acids 27-181 of human FLT3LG
recommended_dilution:WB 1:1000
predicted_size:26.4 kDa
buffer:PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
host_species:Mouse
applications:IF, WB
reactivities:Human
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文献和实验The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
The Purification of Large Numbers of Antigen Presenting Dendritic Cells from Mouse Spleen
immunofluorescent labeling and flow cytometric cell sorting. If the availability of mice is limiting, our protocol can cater for DC numbers boosted by the administration of fms-like tyrosine kinase 3 ligand (Flt3L), directly via subcutaneous injection
a method of isolating DC from the mouse that involves efficient extraction from tissues, followed by the selection of the lightest density cells, then depletion of non-DC through a cocktail of monoclonal antibodies and anti-immunoglobulin magnetic beads
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