- ￥1999
- null
- TA422513
- 2025年07月08日
- Rabbit
- null
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![GSK3 alpha (GSK3A) Rabbit Monoclonal Antibody [Clone ID: R07-3A2]](https://img1.dxycdn.com/p/s14/2025/0217/601/8427578297774731981.png!wh200)
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
- 克隆性：
Monoclonal
- 适应物种：
null
- 宿主：
Rabbit
- 规格：
100 ul
description：c-Maf Antibody
symbol：MAF
synonyms：Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; c-MAF; c-maf proto-oncogene; cMaf; MAF; MGC71685; Proto-oncogene c-Maf
locusid：4094
Concentration：lot specific
sub_category：Primary Antibodies
uniprot_id：O75444
Applications：IHC
CNdelivery：2周
RecommendDilution：IHC 1:50~1:200
Reactivity：Human, Mouse, Rat
Immunogen：A synthesized peptide derived from human c-Maf
Purification：Affinity-chromatography
Conjugation：Unconjugated
ProteinFamilies：null
ProteinPathways：null
GeneName：MAF bZIP transcription factor
Background：Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element.
Buffer：Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
isotypes_filter：IgG
predicted_size：kDa
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文献和实验Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
monoclonal antibody production fusion
, 50 ml + NaOH, 1N, 100 µl-> heat to 37ѓC, add ddH2 O up to 250 ml 上一篇：Antibody Conjugation Protocol 下一篇：Expanding Hybridoma Clones
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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