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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 克隆性:
Monoclonal
- 适应物种:
null
- 宿主:
Rabbit
- 规格:
100 ul
description:Phospho-PTP1B (Ser352) Rabbit mAb
symbol:PTPN1
synonyms:Protein-tyrosine phosphatase 1B; PTP-1B; PTP1B; PTPN1; Tyrosine-protein phosphatase non-receptor type 1
locusid:5770
Concentration:lot specific
sub_category:Primary Antibodies
uniprot_id:P18031
Applications:ICC/IF, IP, WB
CNdelivery:2周
RecommendDilution:WB: 1/500-1/1000
IF: 1/50-1/200
IP: 1/20
Reactivity:Human
Immunogen:A synthetic phosphopeptide corresponding to residues surrounding Ser352 of human PTP1B (Phosphorylated)
Purification:Affinity Purified
Conjugation:Unconjugated
ProteinFamilies:null
ProteinPathways:null
GeneName:protein tyrosine phosphatase, non-receptor type 1
Background:null
Buffer:50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
isotypes_filter:IgG
predicted_size:Calculated MW: 50 kDa; Observed MW: 50 kDa
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文献和实验Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)
). Day 2 14. If you are not using rabbit polyclonal antibodies (ie. monoclonal antibodies, ect), add 1 ug of an appropriate secondary antibody (rabbit) and incubate for an additional 1 hour. 15. Add
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
although other techniques can be used. The choice of immobilized antibody binding protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b . Protein G binds
population is termed monoclonal . It recognizes a single epitope on the antigenic protein. Antibody producing B lymphocytes can be isolated from the spleen or from lymph nodes. However, they have a finite life span in culture, i.e
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![GCLC Rabbit Monoclonal Antibody [Clone ID: R08-2F5]](https://img1.dxycdn.com/p/s14/2025/0217/601/8427578297774731981.png!wh200)

