ROC1 (RBX1) Rabbit Monoclonal

ProductName：ROC1 (RBX1) Rabbit Monoclonal Antibody [Clone ID: R07-3F6]
description：RBX1 Rabbit monoclonal Antibody
symbol：RBX1
synonyms：BA554C12.1; MGC1481; MGC13357; RNF75; ROC1
locusid：9978
Concentration：lot specific
sub_category：Primary Antibodies
uniprot_id：P62877
Applications：IF, IHC, IP, WB
CNdelivery：2周
RecommendDilution：WB: 1/1000
IHC: 1/500
ICC/IF: 1/50
IP: 1/20
Reactivity：Human, Mouse, Rat
Immunogen：A synthetic peptide of human ROC1
Purification：Affinity Purified
Conjugation：Unconjugated
ProteinFamilies：null
ProteinPathways：null
GeneName：ring-box 1
Background：Swiss-Prot Acc.P62877.E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair (PubMed:10230407, PubMed:10579999, PubMed:15983046, PubMed:16678110, PubMed:19112177, PubMed:19679664, PubMed:23455478, PubMed:27565346, PubMed:29769719, PubMed:11961546, PubMed:22748924). CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (PubMed:27565346). The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Recruits the E2 ubiquitin-conjugating enzyme CDC34 to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.
Buffer：50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
isotypes_filter：IgG
predicted_size：Calculated MW: 12 kDa; Observed MW: 12 kDa