GEN1 Rabbit Monoclonal Antibod

ProductName：GEN1 Rabbit Monoclonal Antibody [Clone ID: R09-1B8]
description：GEN1 Rabbit monoclonal Antibody
symbol：GEN1
synonyms：DKFZp781F0986; FLJ40869; Gen
locusid：348654
Concentration：lot specific
sub_category：Primary Antibodies
uniprot_id：Q17RS7
Applications：WB
CNdelivery：2周
RecommendDilution：WB: 1/1000
Reactivity：Human, Mouse, Rat
Immunogen：A synthetic peptide of human GEN1
Purification：Affinity Purified
Conjugation：Unconjugated
ProteinFamilies：null
ProteinPathways：null
GeneName：GEN1, Holliday junction 5' flap endonuclease
Background：Swiss-Prot Acc.Q17RS7.Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation (PubMed:19020614, PubMed:26682650). Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction (PubMed:26578604). Efficiently cleaves both single and double HJs contained within large recombination intermediates. Exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA (PubMed:28049850). Has also endonuclease activity on 5'-flap and replication fork (RF) DNA substrates (PubMed:26578604).
Buffer：50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
isotypes_filter：IgG
predicted_size：Calculated MW: 103 kDa; Observed MW: 103 kDa