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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 克隆性:
Monoclonal
- 适应物种:
null
- 宿主:
Rabbit
- 规格:
100 ul
description:BTF3 Rabbit monoclonal Antibody
symbol:BTF3
synonyms:BETA-NAC; BTF3a; BTF3b; NACB
locusid:689
Concentration:lot specific
sub_category:Primary Antibodies
uniprot_id:P20290
Applications:IHC, IP, WB
CNdelivery:2周
RecommendDilution:WB: 1/1000
IHC: 1/50
IP: 1/20
Reactivity:Human, Mouse, Rat
Immunogen:Recombinant protein of human BTF3
Purification:Affinity Purified
Conjugation:Unconjugated
ProteinFamilies:null
ProteinPathways:null
GeneName:basic transcription factor 3
Background:Swiss-Prot Acc.P20290.When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
Buffer:50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
isotypes_filter:IgG
predicted_size:Calculated MW: 22 kDa; Observed MW: 22 kDa
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文献和实验Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)
). Day 2 14. If you are not using rabbit polyclonal antibodies (ie. monoclonal antibodies, ect), add 1 ug of an appropriate secondary antibody (rabbit) and incubate for an additional 1 hour. 15. Add
population is termed monoclonal . It recognizes a single epitope on the antigenic protein. Antibody producing B lymphocytes can be isolated from the spleen or from lymph nodes. However, they have a finite life span in culture, i.e
confirmation. A. Protein microarray hybridization with 8F1. The OriGene overexpression protein microarray chip was immunostained with the most commonly used 8F1 monoclonal anti-ERCC1 antibody. The positive reactive proteins are highlighted with red arrows
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