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本产品是一款专为PAGE凝胶自动化制备而设计的仪器。本产品可高效制备4%~20%各种浓度的梯度胶,其分离效果显著优于传统固定浓度凝胶;使用时无需提前配置制胶液,各组分试剂在仪器内部实时混匀,有效避免了制胶液提前凝固的问题,实现了连续制胶的便利性;内置微升级超高精度柱塞泵,可精确控制各试剂的体积,从而确保提供高质量的凝胶。此外,本产品还具备自动清洗功能,无需手动清洗仪器管路,避免接触有毒试剂。
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文献和实验Casting Immobilized pH Gradients (IPGs)
are obtained through the copolymerization of acidic and basic acrylamido derivatives of different pKs within a polyacrylamide matrix ( 1 , 2 ) (Fig. 1 ). The pH gradient may be devised by computer modeling either with a linear or with an exponential course
Using the Automated DNA Sequencer
. In this chapter, we describe DNA sequencing using the first commercially available fluorescent instrument, the Applied Biosystems, Inc. Model 373A Automated DNA Sequencer. This sequencer, originally introduced in 1987 as the Model 370A, utilizes a multispectral
Mutation Analysis of Cancer Using Automated Sequencing
(ssCP) (1 ), protein truncation test (PTT) (2 ), heteroduplex analysis (HDA) (3 ), temperature gradient gel electrophoresis (TGGE) (4 ), denaturing gradient gel electrophoresis (DGGE) (5 ), and more recently, denaturing high-performance liquid
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