60105-382-B赛默飞150mg无水硫酸镁, 5mg

Protocol for Trichloroacetic Acid (TCA) Precipitation

and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish

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SIGMA公司）。 　　环己烷和乙腈为色谱纯（美国Fisher公司）；其余试剂均为分析纯（北京化学试剂厂）。16种三嗪类类除草剂标准品（农业部农药鉴定所）分别用乙腈配制成200 mg/L储备液，采用乙腈稀释成4 mg/L的16种混合标准溶液。国产GCB，0.086～0.154 mm粒径，STH�2型（吉林吉炭集团研究所）；空SPE柱管（6 mL）和HXN�SPE柱（60 mg，3 mL）（北京艾杰尔科技有限公司）；C18�SPE柱（500 mg，6 mL）、Florisil�SPE柱（500

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analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2