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- 文献和实验
- 技术资料
- 保存条件:
干燥,-80℃储存;特低温运输
- 英文名:
VTP-27999 Hydrochloride
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
1264191-73-2
- 规格:
V656233-1ml
中文名:VTP-27999 Hydrochloride
英文名:VTP-27999 Hydrochloride
纯度:10mM in Water
货号:V656233
Cas号:1264191-73-2
存储温度:干燥,-80℃储存
运输条件:特低温运输
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文献和实验Protocol for First-strand cDNA Synthesis
the mix at 70℃for 5 minutes and chill on ice.Add the following in the order indicated:5X reaction buffer 4µl,10mM 4 dNTP mix 2µl (1.0mM - final concentration),ribonuclease inhibitor 20u,deionized water (nuclease free) to 19µl.Incubate at 37℃for 5 minutes
by adding 1µl Rnase A (10mg/ml) and 20µl of 5M NaCl. Incubate the samples in a 65° water bath or heat block for 4-5 hours to overnight (note 5); ;After the reverse cross-linking the elutes are treated with 10µl of 0.5M EDTA, 20µl of 1M Tris-HCL, pH
Protocol for First-strand cDNA Synth
5X reaction buffer 4µl, 10mM 4 dNTP mix 2µl (1.0mM - final concentration), ribonuclease inhibitor 20u, deionized water (nuclease free) to 19µl. Incubate at 37°C for 5 minutes. If random primer
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