Niaprazine，27367-90-4，10mM in

制备高效率感受态

后, 用1/3体积的冰冷TB溶液悬浮, 在冷10分钟再次离心回收菌体用1/12.5体积的TB悬浮, 添加最终浓度为7%的DMSO, 再冷却10分钟0.1-1毫升分装, 直接在液氮中冻上.在液氮或-80度保存. TB溶液*TRANSFORMATION BUFFER PIPES 3.0G 10mM CaCl2.2H2O 2.2g 15mM KCl 18.6g 250mM add water up to 950ml 用 5N KOH

In Situ HYBRIDIZATION TO TISSUE SECTIONS

hr, etc.) 2.5 ml 8% paraformaldehyde 0.5 ml 1X PBS 2.0 ml DMSO Add 5 ml heptane and shake vigorously for 20' (Buffer A) or 30' (Buffer B). Transfer to 10 ml 90% MeOH/EGTA (9ml MeOH + 1 ml 50mM EGTA

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

gr/l Difco bacto agar. Hypoclorite solution: for 50 ml: 4ml Na Hypoclorite 15% 255l Tween-20 water to 50ml   cDNA PROBES mRNA 1-5ug H2O 50ul oligoDt (10mg/ml) 2ul heat 90C 2'', ice 5xRT buffer 20 10mM