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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
Malic enzyme inhibitor ME1
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
522649-59-8
- 规格:
M655953-1ml
英文名:Malic enzyme inhibitor ME1
纯度或规格:10mM in DMSO
SPU:M655953
CAS:522649-59-8
存储温度:-80℃储存
运输条件:特低温运输
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文献和实验for preparing assay reagents, performing HTS for USP14 inhibitors, and carrying out post?HTS analysis. Curr. Protoc. Chem. Biol. 4:311?330 © 2012 by John Wiley & Sons, Inc. Keywords: 26S proteasome; USP14; deubiquitinating enzyme; high
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
are then cloned into a vector harboring the same restriction enzyme site overhangs that were generated in the enzyme digestion step of the 3C portion of the protocol. The clones are further screened by digestion with the same restriction enzyme. Ideally
) -10X RT Buffer (500 mM Tris-HCl, pH 8.3, 750 mM KCl, 30mM MgCl2 50 mM DTT) -Random decamers (50μM) -dNTP (2.5mM each dNTP) -RNase Inhibitor (10 units/μl) Before you start: •Place RNase Inhibitor and Reverse Transcriptase ON ICE directly
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