IPR-803，892243-35-5，10mM in DM

In Situ HYBRIDIZATION TO TISSUE SECTIONS

dechorionated embryos to either buffer A or B (depending on their age): Buffer A (0-2, 1-3 hr embryos) 2.5 ml 8% paraformaldehyde (fresh) 2.0 ml 1X PBS 0.5 ml DMSO - or - Buffer B (2-4, 3-5

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

gr/l Difco bacto agar. Hypoclorite solution: for 50 ml: 4ml Na Hypoclorite 15% 255l Tween-20 water to 50ml   cDNA PROBES mRNA 1-5ug H2O 50ul oligoDt (10mg/ml) 2ul heat 90C 2'', ice 5xRT buffer 20 10mM

常用分子生物学实验方法

2.7.1PCR 鉴定体系 10X Taq buffer 3 ul 4X dNTP (10mM) 0.5 ul Taq DNA polymerase(5U/ul) 0.2ul(1U) 10uM引物 (双向) 各1ul 50%DMSO(which is optional) 1ul 模板：过夜菌液 / 挑单克隆 2ul 加灭菌水至 30ul 2.7.2 PCR 鉴定程序： 94℃ 5min 94℃ 30s 30cycle 55℃ 30s 72℃ 3min 72℃