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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
BGC-20-1531 free base
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
736183-35-0
- 规格:
B656774-1ml
英文名:BGC-20-1531 free base
纯度或规格:10mM in DMSO
SPU:B656774
CAS:736183-35-0
存储温度:-80℃储存
运输条件:特低温运输
查看阿拉丁官网此产品相关对应页面:
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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
target is very low. For example, there is a 1/4 chance (4-1) of finding an A, G, C or T in any given DNA sequence; there is a 1/16 chance (4-2) of finding any dinucleotide sequence (eg. AG); a 1/256 chance of finding a given 4-base sequence
In Situ HYBRIDIZATION TO TISSUE SECTIONS
. Before using, boil 2' and chill on ice. Riboprobes (35S) Plasmid templates should be RNase-free CsCl-banded DNA. Linearize DNA with an enzyme that cleaves downstream of the insert. Digest with 200mg/ml Proteinase K (30' @ 37 degrees C), phenol
can be detected following 35 cycles of PCR amplification. However, if the target is rare or if only a small amount of starting material is available, it may be necessary to increase the number of cycles to 45 or 50. Q: What advantages does the Access RT
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