Anamorelin hydrochloride，86199

常用分子生物学实验方法

)，且两条引物的Tm 相差不超过4℃。引物之间及引物本身避免形 成稳定的二聚体或发夹结构，引物与模板不能有任何错配。 forward primer 5’加:GGCCAATCCGGCC reverse primer 5’加:GGCCTCTAAGGCC 2.2 PCR 2.2.1 PCR 反应体系 模扳(100ng/ul 质粒) 2.00μl 4dNTP(10mM) 1.00μl 10×PCR Buffer 5.00μl MgCl2(25mM) 5.00μl

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

411400 (Boehringer). For 10mM stock, weigh out 1mg (light sensitive!), add 27ul DMSO to solubilize, then add 12ul 3M NaOAc, pH5, mix, then 275ul H2O, Don''t make up large amountrs as this is not very stable, even at -80C. Diluted luciferin solution

Purification of recombinant sBRF M166L

J.Movius,K,Coachman,and S.Hahn (Hahn Lab) last modified 10/9/00 Induction of BRF in bacteria and purification on Ni-NTA agarose Transform BRF plasmid into strain BL21 DE3 (pLysS)or JM25 (DE3 containing the Arg tRNA over expression plasmid