AP-III-a4 hydrochloride，207001

Northern Blot: Glyoxal/DMSO

the gel: dissolve 0.8g agarose in 100mL of 10mM NaPO4 pH 7.0 (5mL of 0.2M NaP04 pH 7.0 + 95mL "DEPC" H2 O). Make sure gel box and comb are RNase-free by treatment with 3% H2 O2 or commericial RNase removal product (e.g. RNase Zap from Ambion).

Yeast Cell Lysates

from stocks to give 50 fold dilution. Example: 20 ul of stock per 1ml buffer. Use IMMEDIATELY after adding protease inhibitors.   SUMEB BUFFER (1% SDS, 8M Urea, 10mM MOPS, pH 6.8, 10mM EDTA, 0.01% bromophenol blue) TO MAKE 100ml: 1.0ml 1M

In Situ HYBRIDIZATION TO TISSUE SECTIONS

dechorionated embryos to either buffer A or B (depending on their age): Buffer A (0-2, 1-3 hr embryos) 2.5 ml 8% paraformaldehyde (fresh) 2.0 ml 1X PBS 0.5 ml DMSO - or - Buffer B (2-4, 3-5